Postal address Edificio Santiago Gascón, Campus del Cristo
Universidad de Oviedo
Postal code
33006
Oviedo, SPAIN

Phone (+34) 985 104211(+34) 985 103565
Fax
(+34) 985 106279
 
 
    COORDINATORS 
    Name : Pilar de la Peña / Francisco Barros
    Mail : pdelapena@uniovi.esfbarros@uniovi.es
 
    RESEARCH INTEREST 

   
  Structure-function relationships and mechanisms  of regulation in potassium channels

    •  Molecular characterization of transduction signaling pathways in eukaryotic cells

 
    TECNOLOGY AND KNOWLEDGE OFFER 

    •
  Isolation and characterization of DNA and RNA.
      Cloning, site-directed mutagenesis and heterologous functional gene expression.
      Microinjection and biochemical or electrophysiological detection of heterologously expressed
         proteins in Xenopus laevis oocytes.
      Functional characterization of proteins (protein-protein interactions, double hybrid
         techniques, inmunoprecipitation and inmunodetection, etc).
      Transient and stable transfection of cultured eukaryotic cells.
      Patch-clamp recording of single channel and macroscopic ionic currents.
      Fluorimetric recording of single cell pH and intracellular calcium levels.
      Imaging system for in vivo FRET measurements in eukaryotic cells.
   
  TIRF microscopy and TIRF/FRET combination at 440, 488 and 514 nm (CFP, GFP and YFP).
   
  Voltage clamp fluorometry (VCF)